This is the research analysis technique that can simultaneously check spatial information and transcriptome information in tissues.
Spatial transcriptome analysis is the latest genetic sequencing technology, which simultaneously assesses the gene expression of cells and the location of the cells. It can directly analyze the gene expression of the tissue section itself without separation into single cells or RNA extraction. By doing so, you can understand the tissue heterogeneity at the same time by obtaining the unique location information of cells in the tissue, as well as the gene expression information. It has the advantage of being applicable to fresh tissue obtained from surgery, FFPE tissue treated with formalin for tissue preservation, in addition to pre-sectioned tissues.
As a CSP (Certified Service Provider) for spatial transcriptomics analysis by 10x Genomics, Macrogen is recognized for quick service delivery and a superior level of performance based on its NGS (Next Generation Sequencing) performance, and is ranked first in Korea and fifth in the world. We are also leading the market with diversified spatial transcriptomics analysis services by rapidly introducing new techniques.
PLATFORM
Visium CytAssist
Explore and Discover spatial organization of gene expression
Visium : Sample Preparation
|
FF (Fresh Frozen) |
FFPE (Formalin Fixed Paraffin Embedded) |
Sectioned slide | |
|---|---|---|---|
| Sample type | OCT (compound) block | Paraffin block | FF / FFPE |
| Section Thickness | 10 μm | 5 μm | FF 10µm / FFPE 5µm |
| RNA Quality | RIN ≥ 7 | DV200 ≥ 50% | FF RIN ≥ 4 / FFPE DV200 ≥ 30% |
| Species | Any organism (poly A tail) | Only Human, Mouse | Only Human, Mouse |
- Visium-FF : Section (Cryotome) - RNA QC & ROI* check - Tissue optimization test
- Visium-FFPE : Section (Microtome) - RNA QC & ROI check - Tissue adhesion test
- Visium CytAssist : RNA QC & ROI check - no tissue optimization, tissue adhesion test
*ROI : Region of interest
Nanostring GeoMx® DSP
Discover Where Biology Happens
| Platform | GeoMx® Digital Spatial Profiler |
|---|---|
| Sample |
FFPE Block (Storage period: 3 years / 35.3mm x 14.1 mm x 4-6 µm / Core Needle Biopsy available) Fresh Frozen Tissue (Store at -80℃, in cutting, within 6 months/ 35.3mm x 14.1 mm x 4-6 µm ) |
| Panel | Human Whole Transcriptome Atlas (hWTA) |