NGS
Next Generation Sequencing
SERVICE

Superior NGS service with a high level of precision and speed
based on world-class analytical infrastructure

NGS (Next Generation Sequencing) is a method for reading a genome by dividing it into many pieces, assembling the obtained sequence pieces and analyzing the
sequence of the entire genome.

NgsIntro

Macrogen is a global leader in precision medicine that is capable of predicting and diagnosing diseases through NGS information-based R&D and collaboration with medical institutions, presenting personalized treatment plans according to genetic characteristics.
There has been rapid progress in technologies related to NGS since its commercialization in 2000, which has produced a large volume of genome information for use in research. Especially since the cost and time for genome analysis gradually decreased, the demand for genome analysis for living organisms including humans has rapidly increased and became available to use in various research. Recently, mostly in the medical field, clinical studies based on NGS are actively conducted and many studies are in progress to identify genetic variance and the functions of new genes. This is expected to speed up the realization of precision medicine that can provide treatments optimized for individual patients based on their genome.

Macrogen produces a large amount of genome information every year using various kinds of equipment, provides quicker and more accurate genome analysis service at an economical price to assist in studies conducted by researchers, and offers a diagnostic service to the public. We provide genome analysis services suitable for research, such as whole genome, exome, transcriptome, epigenome, metagenome, and single cell multi-omics sequencing and bioinformatics results based on the data. Offering a total genomic solution to researchers all over the world based on our extensive experiences and know-how accumulated over the years, Macrogen leads the field of precision medicine by utilizing NGS technology

SERVICE

Whole Genome Sequencing

Whole Genome Sequencing (WGS) is a procedure for reading the entire genome and analyzing related genetic information.

Information about mutations obtained through WGS is used for genetic discovery and personalized medicine research in relation to disease. Currently, we have more than 18,000 research institutes as clients in 153 countries around the world, as well as world-class analytical infrastructure, including WGS analysis facilities for more than 300,000 people per year and computing systems that can store up to 30 PB. Based on these resources, we are opening the door to a new era of genome information-based precision medicine powered by big data.

Whole Genome Sequencing

Whole Genome Resequencing

Resequencing is a procedure that allows variation analysis, such as SNP (Single Nucleotide Polymorphism), InDel (Insertion & Deletion), CNV (Copy Number
Variation), and SV (Structural Variation) based on reference genomes.

Sequencing Platform

  • HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000
  • NextSeq 500

Whole Genome De novo Sequencing

This is a procedure that can uncover information about the entire genome of microorganisms or animals and plants that still isn't known.

Sequencing Platform

  • HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000
  • NextSeq 500 / MiSeq
  • PacBio Sequel I & Sequel II
  • PromethION

Whole Exome Sequencing

WES (Whole Exome Sequencing) is a procedure for selective analysis in an exon area known to be where genes exist.

It is a more effective and economical way of performing research than whole genome resequencing because it selectively analyzes only the genes obtained from current studies. It also supports whole exome sequencing using Capture Kit (Agilent SureSelect Exome Capture kit, Truseq Exome Enrichment Kit,
Twist Human Core Exome Kit) and provides mutation analysis for SNP and InDel.

Whole Exsome Sequencing

Main Capture Kit

  • Agilent SureSelect Exome Capture kit
  • Truseq Exome Enrichment Kit
  • Capture the whole exon and provide mutation analysis for SNP and InDel as well.

Sequencing Platform

  • HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000
  • NextSeq 500

Transcriptome Sequencing

As a technique for analyzing RNA obtained from gene expression, it allows checking the difference in expression profile and various mutation information between samples.

As one of the most frequently used techniques conducted with NGS, it requires a high level of technical skill such as sample preparation, storage, and processing
due to the unstable specificity of RNA.
Based on the expression profile and mutation information between samples, new insights into gene possible research and expression regulation can be obtained,
and it can be applied to biomarker development, diagnosis, and monitoring of diseases.
Macrogen is equipped with a wide range of library kits, NGS systems, and computing systems to meet the needs of staff related to transcriptome sequencing,
based on years of experience.

TranscriptomeSequencing소개이미지

mRNA / Total RNA Sequencing

The exact data value can be known through the mRNA expression value identified based on the read. It can be used for research such as novel transcriptome,
fusion gene, alternative splicing, and mutation information as well as inter-gene expression profiles. Recently, studies on gene expression
at the single cell level is also being actively carried out.

Library Construction Kit

Illumina

  • TruSeq RNA
  • TruSeq stranded mRNA
  • TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat
  • TruSeq Stranded Total RNA with Ribo-Zero Gold Human/Mouse/Rat
  • TruSeq Stranded Total RNA with Ribo-Zero Gold Human/Mouse/RatTruS
  • TruSeq Stranded Total RNA with Ribo-Zero Globin Human/Mouse/Rat
  • TruSeq Stranded Total RNA Sample Preparation Kits with Ribo-Zero Plant
  • TruSeq Stranded Total RNA (NEB Microbe)
  • ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre)
  • TruSeq RNA Access
  • TruSeq Total RNA with Ribo-Zero
  • TruSeq mRNA (Insert size)
  • TruSeq Total RNA with Ribo-Zero (Insert size)

Others

  • SMARTer Universal Low RNA library
  • SMARTer Ultra Low Input RNA library
  • SMARTer Stranded RNA library

Small RNA Sequencing

In addition to mRNA from which genes are transcribed, non-coding RNA that does not contain gene information, especially small RNA, has been found to be key
factors in gene expression control and are actively studied. By analyzing the key types of the small RNA family, miRNA, siRNA, piRNA and snRNA, we can gain insights
into gene expression control and apply them to the development and diagnosis of biomarkers.

Library Construction Kit

Illumina

  • Small RNA library

Others

  • SMARTer Universal Low RNA library
  • QIAseq miRNA library
  • NEBNext Small RNA library

Sequencing Platform

  • HiSeq 2500 / HiSeq 4000
  • NextSeq 500

Isoform Sequencing

For genes composed of exons and introns, new transcripts continue to be discovered by the combination of exon regions that are expressed. Isoform sequencing using a long read platform allows us to identify full-length transcripts that have not been revealed before, so it is gaining attention in regard to functional studies of gene.


Exosomal RNA Sequencing

An exosome is a cell-derived endoplasmic reticulum of less than 200nm, and it contains various types of biomolecules including proteins, DNA, and RNA derived from the origin cell. By using these characteristics, it can be used as a biomarker for diagnosis or for disease monitoring. Recently, the fields of application for treatment, as well as cosmetic applications, are broadening.


Epigenome Sequencing

This is a technique for determining whether the function of a specific gene is properly expressed by external environmental factors.

The key epigenetic regulatory mechanisms for gene transcription include DNA methylation and histone protein acetylation, and they can be analyzed more effectively using the NGS technique. This is a service with a strong chance of lowering the quality compared to Whole Genome Sequencing since the analysis proceeds through modification of the DNA sample with such substances as Bisulfite. Macrogen is capable of providing high-quality analytical data based on NGS performance that is ranked first in Korea and fifth in the world, and ATAC Sequencing is currently the only service available in the country.

Epigenome Sequencing

Methylation Sequencing

WGBS (Whole Genome Bisulfite Sequencing) is a key NGS technique that can check methylation. This DNA processing technique uses sodium bisulfite, a substance that converts unmethylated cytosine to uracil, to observe the level of methylation of DNA by analyzing the nucleotide sequence afterward.


Single Cell Muti-omics analysis

Macrogen provides personalized single cell multi-omics analysis services based on extensive experience and innovation.

Single-cell gene sequencing is used to analyze gene expression profile and genetic changes in a single cell, which is a functional and structural unit of an organism. As it becomes possible to determine cellular heterogeneity, rare cell population, cellular differentiation, chromatin heterogeneity, immune profile, and surface protein profile in a single cell unit, this technique is applied to various fields of research including selection of cell treatments, stem cells, and cancer development and progression. Macrogen quickly provides a high level of genetic sequencing data based on its ability to perform NGS (next generation sequencing), which ranks first in Korea and fifth in the world. Furthermore, we are leading the market by diversifying single-cell sequencing services, thanks to industry-leading genome sequencing expertise accumulated over 24 years and investment in big data and unexplored areas.

Single Cell Muti-omics analysis소개이미지

single cell RNA sequencing

Single cell expression analysis

single nucleus RNA sequencing

Analysis of gene expression after isolation of nucleus from samples that are difficult to progress to single cells

single cell immune profiling

Single cell TCR and BCR gene expression analysis

single cell surface protein profiling

Analysis of proteins located on the surface of single cells

single cell ATAC sequencing

Analysis of single-nucleus DNA's accessibility

single cell Multiome sequencing

Simultaneous analysis of single-nucleus DNA's accessibility and gene expression

With the advent of the single-cell sequencing technique, it became possible to analyze trace amounts of cellular genes and those expressed in small amounts, which cannot be examined through conventional gene analysis. As a result, we are able to gain insights into individuals' environment, genetics, and biological characteristics in detail, allowing us to get one step closer to realizing ultra-precision medicine that can predict, prevent, and diagnose diseases. Macrogen has carried out the most single cell sequencing projects in Korea, and is the only CSP (Certified Service Provider) in the country for single cell gene expression and spatial gene expression services. In addition, we are leading the market by diversifying single-cell sequencing services by introducing innovative single-cell sequencing techniques.


ATAC analysis

ATAC is a technique used to analyze accessible DNA regions through NGS (Next Generation Sequencing).

Macrogen provides a high level of genetic sequencing data quickly based on its NGS (next generation sequencing) performance, which ranks first in Korea and fifth in the world, and supports personalized sample tests. By assessing the open state of closed chromatin condensed into histone proteins for gene expression, the accessible region of RNA polymerase can be inferred, or the transcription factor binding site and nucleosome (nucleosome) location information can be acquired. In order for the genetic information stored in DNA to actually play a role, it should be expressed as a transcript (RNA). The DNA expression is controlled by changes in the DNA sequence itself, but it can also be regulated by changes in the helix structure or changes in surrounding proteins without changing the DNA sequence. The study of changes in gene function through structural changes or changes in surrounding proteins without changes in the DNA sequence is known as epigenetics.

ATAC analysis

Macrogen is the only Korean company that provides the ATAC (Assay for Transposase-Accessible Chromatin) service that analyzes the DNA's open structure through NGS. By assessing the sequence of the regions where the condensed DNA is released for the RNA transcription process through ATAC analysis, it is possible to check how transcription is controlled by RNA, even if the DNA sequences are the same.


Spatial Gene Expression analysis

This is the latest research analysis technique that can simultaneously check tissue spatial information and genomic expression information.

Spatial gene expression analysis is the latest genetic sequencing technology, which simultaneously assesses the genetic information of cells and the location of the cells. It can directly analyze the gene expression of the tissue itself without separation into single cells or RNA extraction. By doing so, you can understand the tissue heterogeneity at the same time by obtaining the unique location information of cells in the tissue, as well as the gene expression information. In addition, it has the advantage of being able to be applied to both the fresh tissue obtained from surgery and FFPE tissue treated with formalin to store the tissue. By analyzing genetic information and tissue location information at once, you can set a direction for treatment or predict efficacy. For example, by checking the genetic information and location of the cancer patient's immune cells and cancer cells, you can determine how effective treatment is, whether cancer cells have metastasized, and the expected prognosis of the treatment.

Spatial Gene Expression analysis

As a CSP (Certified Service Provider) for spatial gene expression service, Macrogen is recognized for quick service delivery and a superior level of performance based on its NGS (Next Generation Sequencing) performance, and is ranked first in Korea and fifth in the world. We are also leading the market with diversified spatial sequencing services by rapidly introducing new spatial genome analysis techniques.


Metagenome Sequencing

This is a method for identifying microbial communities in different environments that allows you to check the diversity of species in the environment by sequencing a set of genomes that exist in various environments at once.

It allows you to find out what species and distribution of a specific group are in the meta sample in which various genomes are mixed, including feces, soil, seawater, and food, and also to understand their interactions and roles. It can also be used as a technique to confirm the microbial community that exists in various environments and assess the diversity and distribution of bacteria and fungi. It also supports the analysis for eukaryotes using the same principle of targeting marker genes. A dedicated team of bioinformatics equipped with superior NGS techniques and equipment delivers results that meet the needs of clients.

Metagenome Sequencing

Metagenome Amplicon Sequencing

After amplifying a specific region of a marker gene such as 16S rRNA or ITS gene and creating an Amplicon library, we analyze the distribution and diversity through sequencing. Eukaryote analysis is also supported using the same principle of targeted marker genes. A dedicated bioinformatics team performs the job. Taxonomic assignment and diversity analysis that guarantees a high level of accuracy and reproducibility through ASV analysis is also possible, as well as advanced analysis that meets statistical analysis and trends.

Sequencing Platform

  • 16S rRNA V3-V4 Regions, 18S rRNA, ITS Regions, Customized regions → Illumina Miseq / Hiseq2500 platform
  • 16S rRNA Full length → PacBio Sequel / Sequel2 platform

Metagenome Shotgun Sequencing

Through targeted sequencing of the entire genome as well as marker gene, this sequencing method enables the analysis of assembly, gene prediction,
and functional annotation in addition to distribution and diversity of microbiota. A dedicated bioinformatics team performs the analysis directly.
For taxonomic assignment, it can increase precision by calculating the normalized abundance using the genome size of the target taxon.

Sequencing Platform

  • Illumina Hiseq XTen / Hiseq 4000 / NovaSeq6000 platform

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