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Whole Exome Sequencing

WES (Whole Exome Sequencing) is a procedure for selective analysis in an exon area known to be where genes exist.

It is a more effective and economical way of performing research than whole genome resequencing because it selectively analyzes only the genes obtained from current studies. It also supports whole exome sequencing using Capture Kit (Agilent SureSelect Exome Capture kit, Truseq Exome Enrichment Kit,
Twist Human Core Exome Kit) and provides mutation analysis for SNP and InDel.

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Transcriptome Sequencing

As a technique for analyzing RNA obtained from gene expression, it allows checking the difference in expression profile and various mutation information between samples.

As one of the most frequently used techniques conducted with NGS, it requires a high level of technical skill such as sample preparation, storage, and processing
due to the unstable specificity of RNA.
Based on the expression profile and mutation information between samples, new insights into gene possible research and expression regulation can be obtained,
and it can be applied to biomarker development, diagnosis, and monitoring of diseases.
Macrogen is equipped with a wide range of library kits, NGS systems, and computing systems to meet the needs of staff related to transcriptome sequencing,
based on years of experience.

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mRNA / Total RNA Sequencing

The exact data value can be known through the mRNA expression value identified based on the read. It can be used for research such as novel transcriptome,
fusion gene, alternative splicing, and mutation information as well as inter-gene expression profiles. Recently, studies on gene expression
at the single cell level is also being actively carried out.

Library Construction Kit

Small RNA Sequencing

In addition to mRNA from which genes are transcribed, non-coding RNA that does not contain gene information, especially small RNA, has been found to be key
factors in gene expression control and are actively studied. By analyzing the key types of the small RNA family, miRNA, siRNA, piRNA and snRNA, we can gain insights
into gene expression control and apply them to the development and diagnosis of biomarkers.

Library Construction Kit

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Isoform Sequencing

For genes composed of exons and introns, new transcripts continue to be discovered by the combination of exon regions that are expressed. Isoform sequencing using a long read platform allows us to identify full-length transcripts that have not been revealed before, so it is gaining attention in regard to functional studies of gene.

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Exosomal RNA Sequencing

An exosome is a cell-derived endoplasmic reticulum of less than 200nm, and it contains various types of biomolecules including proteins, DNA, and RNA derived from the origin cell. By using these characteristics, it can be used as a biomarker for diagnosis or for disease monitoring. Recently, the fields of application for treatment, as well as cosmetic applications, are broadening.

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Epigenome Sequencing

This is a technique for determining whether the function of a specific gene is properly expressed by external environmental factors.

The key epigenetic regulatory mechanisms for gene transcription include DNA methylation and histone protein acetylation, and they can be analyzed more effectively using the NGS technique. This is a service with a strong chance of lowering the quality compared to Whole Genome Sequencing since the analysis proceeds through modification of the DNA sample with such substances as Bisulfite. Macrogen is capable of providing high-quality analytical data based on NGS performance that is ranked first in Korea and fifth in the world, and ATAC Sequencing is currently the only service available in the country.

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Methylation Sequencing

WGBS (Whole Genome Bisulfite Sequencing) is a key NGS technique that can check methylation. This DNA processing technique uses sodium bisulfite, a substance that converts unmethylated cytosine to uracil, to observe the level of methylation of DNA by analyzing the nucleotide sequence afterward.

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ATAC analysis

ATAC is a technique used to analyze accessible DNA regions through NGS (Next Generation Sequencing).

Macrogen provides a high level of genetic sequencing data quickly based on its NGS (next generation sequencing) performance, which ranks first in Korea and fifth in the world, and supports personalized sample tests. By assessing the open state of closed chromatin condensed into histone proteins for gene expression, the accessible region of RNA polymerase can be inferred, or the transcription factor binding site and nucleosome (nucleosome) location information can be acquired. In order for the genetic information stored in DNA to actually play a role, it should be expressed as a transcript (RNA). The DNA expression is controlled by changes in the DNA sequence itself, but it can also be regulated by changes in the helix structure or changes in surrounding proteins without changing the DNA sequence. The study of changes in gene function through structural changes or changes in surrounding proteins without changes in the DNA sequence is known as epigenetics.

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Metagenome Sequencing

This is a method for identifying microbial communities in different environments that allows you to check the diversity of species in the environment by sequencing a set of genomes that exist in various environments at once.

It allows you to find out what species and distribution of a specific group are in the meta sample in which various genomes are mixed, including feces, soil, seawater, and food, and also to understand their interactions and roles. It can also be used as a technique to confirm the microbial community that exists in various environments and assess the diversity and distribution of bacteria and fungi. It also supports the analysis for eukaryotes using the same principle of targeting marker genes. A dedicated team of bioinformatics equipped with superior NGS techniques and equipment delivers results that meet the needs of clients.

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Metagenome Amplicon Sequencing

After amplifying a specific region of a marker gene such as 16S rRNA or ITS gene and creating an Amplicon library, we analyze the distribution and diversity through sequencing. Eukaryote analysis is also supported using the same principle of targeted marker genes. A dedicated bioinformatics team performs the job. Taxonomic assignment and diversity analysis that guarantees a high level of accuracy and reproducibility through ASV analysis is also possible, as well as advanced analysis that meets statistical analysis and trends.

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Metagenome Shotgun Sequencing

Through targeted sequencing of the entire genome as well as marker gene, this sequencing method enables the analysis of assembly, gene prediction,
and functional annotation in addition to distribution and diversity of microbiota. A dedicated bioinformatics team performs the analysis directly.
For taxonomic assignment, it can increase precision by calculating the normalized abundance using the genome size of the target taxon.

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