Customized GEM (Genetically Engineered Mouse) services
and CRISPR(Clustered Regularly Interspaced Short
Palindromic Repeats) technologies for genome editing
Macrogen produces and supplies genetically engineered mice (GEM) based on CRISPR.
GEM (Genetically Engineered Mouse) is a laboratory mouse that has had certain genes inserted, removed, or modified using gene-editing technologies. Such mice are widely used in biological and medical research to identify the functions of human genes. Macrogen’s main GEM service offers Transgenic Mice and Knock-Out/Knock-In mice. A Transgenic Mouse is one in which a specific gene has been inserted to study the function of that gene. A Knock-Out Mouse is a genetically engineered mouse in which the expression of a target gene has been suppressed by removing a specific gene, and a Knock-In Mouse is a genetically engineered mouse in which the expression of a target gene can be adjusted or explored by modifying a certain gene. Macrogen also offers a wide range of other GEM-related services including mass reproduction, mouse line recovery, embryo freezing and reproduction to live, and KO/TG mouse maintenance. To ensure process efficiency, our genetically engineered mice are produced after a thorough experiment design process with our clients. We provide a customized partial process service to meet various client requirements, and our strict quarantine system ensures that all mice are maintained and supplied in a specific pathogen free (SPF) state.
CRISPR-Cas9, Macrogen's flagship service, is made by attaching a guide RNA that functions as a guide and Cas9 protein that cuts DNA. In principle, the position to cut and edit the DNA sequence varies, depending on the design of the guide RNA that finds the target DNA. Macrogen is developing gene editing technology with Seoul National University Hospital and the most prestigious research institutes around the world, including the Broad Institute co-founded by MIT (Massachusetts Institute of Technology) and Harvard University. Furthermore, we have secured over 50 CRISPR gene editing technologies owned by the Broad Institute in America.
We produce and supply KI/KO mice quickly at low cost based on CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats).
CRISPR gRNA Synthesis
We supply gRNA design, gRNA synthesis, and synthesized gRNA for specific genes. CRISPR/Cas9 is considered as the most advanced technology to modify/edit genes, and Macrogen synthesizes and provides CRISPR gRNA that researchers need. This allows them directly perform corrections of such genes as KO or KI on cells. Macrogen is developing gene editing technology with Seoul National University Hospital and the most prestigious domestic and overseas research institutes, including the Broad Institute co-founded by MIT (Massachusetts Institute of Technology) and Harvard University. We have also managed to secure more than 50 CRISPR gene editing technologies owned by the Broad Research Institute in the United States, and exclusively introduced the "drug-induced CRISPR-Cas9 recombination vector technology” developed by Professor Lee Eun-Joo and Kim Hyo-Soo from the Cell Therapy Commercialization Center at Seoul National University Hospital. The Macrogen GEM Division searches for and delivers gRNA candidates that meet the researcher's needs for free.
CRISPR Validated gRNA
We supply gRNA design, gRNA synthesis, and activity-measured gRNA for specific genes.
CRISPR gRNA Plasmid
CRISPR KI/KO Mouse
Genetically Engineered Mouse
We provide GEM services for the production and supply of genetically engineered mice.
This is a mouse production service in which a specific gene has been removed. KO (knock-out) is a technology that produces individuals with new traits by removing specific genes. This allows us to understand the function of specific genes in vivo and to identify genetic mutations that are associated with specific diseases. Macrogen has superior expertise and know-how to secure the mice that researchers need, based on extensive experience in the field.
Conditional Knock-out (cKO) Mouse
Knock-in (KI) Mouse
Transgenic (TG) Mouse
Macrogen uses IVF (In Vitro Fertilization) technology to offer a mass reproduction service.
We offer additional testing services in various scales tailored to the needs of our clients.
Embryo freezing & Reproduction to Live
Macrogen operates a GEM bank system while also using cryo-sperm and embryos to provide embryo freezing and reproduction services.
If it is difficult to manage disease model mice, then we provide a fertilized egg freezing service where we freeze their fertilized eggs so they can be freely moved and sold. Our technological prowess enables us to offer long-term storage services for our clients’ GEM.
Mouse Line Recovery
This is a service for breeding and maintaining disease model mice at SPF facilities. We provide IVF (In Vitro Fertilization) and ET (Embryo Transfer) services for mice with a low reproducibility or clients who need lineage maintenance of GEM. Our staff at the SPF facilities are in charge of maintaining the cycle and reproduction schedule for clients. Detailed information on mouse management is provided once every three months, so our clients can use the service with confidence.