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  • Service
  • Microarray
  • Gene Expression
  • Illunina Expression

Full-length 50-mer probe provide better selectivity and sensitivity
High feature density enables low sample and reagent volumes
Feature redundancy (30X average) delivers high-confidence results
Low cost per sample allows bigger experimental scale

Illumina expression beadarray
Products Array Contents Special Analysis
Human
  • HumanHT-12 v4.0
47,000 human targets 12 sample / Chip
Mouse
  • MouseWG-6 v2.0
45,000 mouse targets 6sample / Chip
  • MouseRef-8 v2.0
25,000 mouse targets 8 sample / Chip

Task Detail
Raw data Extraction of signal data from image data
Check the experimental control summary
Annotation information
Data QC Data quality check
Filtering by metrics based on quality assessment
Normalization
Data Transform
Data analysis of DE
(Probe/Gene)
Basic statistics(Fold change, group mean, sd etc.)
Identifying differentially expressed genes(T-test, LPE test, ANOVA etc.)
Multiple testing correction (FDR, Bonferroni etc.)
Clustering Analysis for DEG ( Hierarchical clustering, K-means etc.)
Functional analysis KEGG pathway, GeneOntology annotation(DAVID, goProfiles, GOstats etc.)
GeneSet Enrichment Analysis
Customized analysis Integrative analysis (mRNA-miRNA, methylation~mRNA, mRNA-CNV etc.)

Total RNA Quantity >2ug (>100ng/ul)
A260/280, A260/230 >1.7
28S/18S rRNA ratio >1.7
volume >10 ul